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Cleaning of the Pancreas and Cannulation of the Pancreatic Duct
 The pancreas is cleaned under a Class 100 Biological Safety Cabinet (BSC). The
organ is placed in a tray containing cold preservation solution and kept submerged during the cleaning process.
Extraneous tissue attached to the pancreas such as spleen, duodenum, and fat are
carefully removed by blunt dissection. The pancreas is cannulated by placement
of a high flow angiocatheter into the pancreatic duct. The cannula is secured into the
pancreatic duct(s) by suturing. The pancreas is quickly dipped into an
antiseptic solution, and subsequently an antibiotic solution, to decontaminate
the organ. The organ is then rinsed in Hank’s Balanced Saline Solution (HBSS),
and placed in a clean tray. |
